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A misfire in cylinder #8 is not a tuning issue. Most likely an ignition issue. Swap the #8 plug, wire, and coil to another cylinder and see if you can get the misfire to occur again. If the misfire moves to another cylinder, you have your answer. If the misfire stays at cylinder #8 it may be an injector. Worse case scenario is that you have a mechanical issue with the engine.
The problems described in your first post are definitely tune related and a misfire will only exacerbate the problem.
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Bolt-ons.
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